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mouse igg1 anti-human cd3  (Bio-Rad)


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    Structured Review

    Bio-Rad mouse igg1 anti-human cd3
    Mouse Igg1 Anti Human Cd3, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+igg1+anti-human+cd3/Mouse+anti+Human+CD31/pm40940876-69-29-39
    Average 94 stars, based on 118 article reviews
    mouse igg1 anti-human cd3 - by Bioz Stars, 2026-09
    94/100 stars

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    Article Title: Determining the efficacy of ExThera Seraph100 blood filtration in patients diagnosed with pancreatic cancer through the liquid biopsy
    Article Snippet: At room temperature, slides were processed in the IntelliPATH FLXTM autostainer (Biocare Medical LLC, Irvine, CA, USA) and were stained with 2.5 μg/mL of a mouse IgG1 anti-human CD31: Alexa Fluor 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, California, USA; RRID:AB_322463), 100 μg/mL of a goat anti-mouse IgG monoclonal Fab fragments (115-007-003, Jackson ImmunoResearch, West Grove, Philadelphia, USA), and were permeabilized with 100% cold methanol.

    Article Title: Longitudinal tracking of circulating rare events in the liquid biopsy of stage III–IV non-small cell lung cancer patients
    Article Snippet: Briefly, cells were fixed with paraformaldehyde prior to incubation with 2.5 ug/ml of a mouse IgG1 anti-human CD31:Alexa Fluor® 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, CA) and 100 ug/ml of a goat anti-mouse IgG monoclonal Fab fragments (115-007-003, Jackson ImmunoResearch, West Grove, PA), permeabilized using 100% cold methanol, followed by an antibody cocktail consisting of mouse IgG1/IgG2a anti-human cytokeratin (CK) 1, 4, 5, 6, 8, 10, 13, 18, and 19 (clones: C-11, PCK-26, CY-90, KS-1A3, M20, A53-B/A2; C2562, Sigma, St. Louis, MO), mouse IgG1 anti-human CK 19 (clone: RCK108, GA61561-2, Dako, Carpinteria, CA), mouse anti-human CD45:Alexa Fluor® 647 (clone: F10-89-4, MCA87A647, AbD Serotec, Raleigh, NC), and rabbit IgG anti-human vimentin (Vim) (clone: D21H3, 9854BC, Cell Signaling, Danvers, MA).

    Article Title: Automated Single-Cell Analysis in the Liquid Biopsy of Breast Cancer.
    Article Snippet: Specifically, the following steps were applied to each slide as detailed previously [40]: • Incubated for 4 h with a conjugate containing the following: # 2.5 μg/mL of a mouse IgG1 anti-human CD31:Alexa Fluor 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, CA, USA); # 100 μg/mL of a goat antimouse IgG monoclonal Fab fragments (115–007–003, Jackson ImmunoResearch, West Grove, PA, USA).

    Article Title: Cancer-related cells and oncosomes in the liquid biopsy of pancreatic cancer patients undergoing surgery
    Article Snippet: Briefly, after fixation with 2% paraformaldehyde, slides are incubated with 2.5 ug/mL of a mouse IgG1 anti-human CD31:Alexa Fluor® 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, CA, USA) and 100 ug/mL of a goat anti-mouse IgG monoclonal Fab fragments (115-007- 003, Jackson ImmunoResearch, West Grove, PA, USA).

    Article Title: Investigation of liquid biopsy analytes in peripheral blood of individuals after SARS-CoV-2 infection
    Article Snippet: Slides are stained with immunofluorescent (IF) markers at room temperature using the IntelliPATH FLXTM autostainer (Biocare Medical LLC, Irvine, CA, USA) with negative and positive controls as previously described and validated ( )., Briefly, samples were stained with the Landscape assay using 2.5 μg/mL of a mouse IgG1 anti-human CD31:Alexa Fluor® 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, CA, USA, RRID:AB_322463), permeabilized with cold methanol, followed by an antibody cocktail consisting of mouse IgG1/Ig2a anti-human cytokeratins (CKs) 1, 4, 5, 6, 8, 10, 13, 18, and 19 (clones: C-11, PCK-26, CY-90, KS-1A3, M20, A53-B/A2, C2562, Sigma, St. Louis, MO, USA, RRID:AB_476839), mouse IgG1 anti-human CK 19 (clone: RCK108, GA61561-2, Dako, Carpinteria, CA, USA), mouse anti-human CD45:Alexa Fluor® 647 (clone: F10-89-4, MCA87A647, AbD Serotec, Raleigh, NC, USA, RRID:AB_324730) and rabbit IgG anti-human vimentin (Vim) (clone: D21H3, 9854BC, Cell Signaling, Danvers, MA, USA, RRID:AB_10829352).

    Article Title: Investigation of liquid biopsy analytes in peripheral blood of individuals after SARS-CoV-2 infection.
    Article Snippet: Slides are stained with immunofluorescent (IF) markers at room temperature using the IntelliPATH FLXTM autostainer (Biocare Medical LLC, Irvine, CA, USA) with negative and positive controls as previously described and validated (Supplemental Fig. S1).23,24 Briefly, samples were stained with the Landscape assay using 2.5 μg/mL of a mouse IgG1 anti-human CD31:Alexa Fluor® 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, CA, USA, RRID:AB_322463), permeabilized with cold methanol, followed by an antibody cocktail consisting of mouse IgG1/Ig2a anti-human cytokeratins (CKs) 1, 4, 5, 6, 8, 10, 13, 18, and 19 (clones: C-11, PCK-26, CY-90, KS-1A3, M20, A53-B/A2, C2562, Sigma, St. Louis, MO, USA, RRID:AB_476839), mouse IgG1 anti-human CK 19 (clone: RCK108, GA61561-2, Dako, Carpinteria, CA, USA), mouse antihuman CD45:Alexa Fluor® 647 (clone: F10-89-4, MCA87A647, AbD Serotec, Raleigh, NC, USA, RRID:AB_324730) and rabbit IgG anti-human vimentin (Vim) (clone: D21H3, 9854BC, Cell Signaling, Danvers, MA, USA, RRID:AB_10829352).

    Article Title: Automated Single-Cell Analysis in the Liquid Biopsy of Breast Cancer
    Article Snippet: Specifically, the following steps were applied to each slide as detailed previously [ ]: Incubated for 4 h with a conjugate containing the following: ○ 2.5 μg/mL of a mouse IgG1 anti-human CD31:Alexa Fluor 647 mAb (clone: WM59, MCA1738A647, BioRad, Hercules, CA, USA); ○ 100 μg/mL of a goat antimouse IgG monoclonal Fab fragments (115–007–003, Jackson ImmunoResearch, West Grove, PA, USA).



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